貨號	規格	價格	上海	北京	武漢	南京
S86653-1mg	99.8%	￥544.00 S86653-1mg ￥544.00 現貨	3 3	-	-	-
S86653-2mg	≥98%	￥816.00 S86653-2mg ￥816.00 2-3天	0 貨期：2-3天	-	-	-
S86653-5mg	99.8%	￥940.00 S86653-5mg ￥940.00 現貨	4 4	-	-	-
S86653-10mg	99.8%	￥1700.00 S86653-10mg ￥1700.00 現貨	6 6	-	-	-
S86653-25mg	99.8%	￥2720.00 S86653-25mg ￥2720.00 現貨	8 8	-	-	-
S86653-50mg	99.8%	￥5100.00 S86653-50mg ￥5100.00 現貨	5 5	-	-	-

產品介紹

6-OAU (GTPL5846) (6-n-octylaminouracil) is an GPR84 (G protein-coupled receptor 84) agonist, with an EC50 value of 105 nM. 6-OAU works as a chemoattractant to both PMNs and macrophages, and amplifies the proinflammatory cytokine IL-8, shows proinflammatory function. 6-OAU also displays anti-bacterial function

產品描述：

靶點：

ERK;GPR

體外研究：

GPR84 gene exhibits high expression in human polymorphonuclear leukocytes (PMNs) and macrophages, 6-OAU acts on proinflammatory function by activitating GPR84. 6-OAU (0.01 nM-0.1 mM; 1 h) activates human GPR84 in the presence of Gqi5 chimera with an EC50 value of 105 nM in HEK293 cells. 6-OAU (0, 6.25, 200 μM; 30 min) stimulates [35S]GTP binding, accumulates phosphoinositides, and induces GPR84-EGFP internalization in a GPR84-dependent manner. 6-OAU (1 nM-1 mM; 1 h) provokes chemotaxis of PMNs in a concentration-dependent manner with an EC50 value of 318 nM. 6-OAU (0-10 μM; 4 h) increases the secretion of IL-8 from LPS-stimulated PMNs. 6-OAU (0-0.4 μM; 16 h) also amplifies TNF-α production from U937 macrophages. 6-OAU (2 μM; 4 h) decreases ERK phosphorylation and MCP-1 protein expression, (2 μM; 48 h) decreases MCP-1 secretion in macrophages. 6-OAU (2 μM; 24 h) reduces ROS production during B. abortus infection in RAW264.7 cells. 6-OAU (2 μM; 0, 30, and 60 min) inhibits adhesion and Brucella uptake in RAW264.7 cells and (2 μM; 30 min) shows anti-infection against Brucella and Salmonella infection. Cell Viability Assay Cell Line: B. abortus Concentration: 0, 0.02, 0.2, 2 μM Incubation Time: 0, 2, 24, 48, 72 hours Result: Decreased B. abortus survivability begin at 48 h with a dose of 2 μM. Western Blot Analysis Cell Line: RAW264.7 cells infected with B. abortus Concentration: 2 μM Incubation Time: 4 hours Result: Reduced ERK phosphorylation and MALT1 expression in RAW264.7 macrophag

體內研究：

6-OAU activates GPR84 and results in making an inflammatory condition through chemokine production and chemotaxis in vivo. 6-OAU (10 mg/kg; i.v.) raises the blood CXCL1 level in rats. 6-OAU (1 mg/mL; s.c.) attracts both PMNs and macrophages into the air pouch. 6-OAU (2 μM, 100 mL/mouse; s.c.) augments resistance to Brucella infection, and reduces bacterial proliferation in spleens and livers. Animal Model: Female Lewis rats(4-week-old) Dosage: 10 mg/kg Administration: Intravenous injection; collecting blood 3 h after injection Result: Increased the elevation of a chemokine, CXCL1 concentration in the serum peaking at 3 h after the injection. Animal Model: Rat air pouch model (4-week-old female rats) Dosage: 1 mg/mL (PBS) Administration: Subcutaneous injection; washing the cavity 4 h after injection Result: Attracted both PMNs and macrophages into the air pouch, peaking at 4 h after the injection. Animal Model: ICR female mice (7-week-old) Dosage: 2 μM (100 μL/mouse) Administration: Oral average; 7 days and another 14 days after treated mouse with B. abortus (2 × 105 CFU /100 μL; i.p.) Result: Reduced bacterial proliferation in the liver and spleen, and decreased IFN-γ but augmented IL-6 serum level.Lowed splenic weight of mice and splenic proliferation.

參考文獻：

1. Suzuki M, et al. Medium-chain fatty acid-sensing receptor, GPR84, is a proinflammatory receptor. J Biol Chem. 2013 Apr 12;288(15):10684-91. 2. Reyes AWB, et al. Immune-metabolic receptor GPR84 surrogate and endogenous agonists, 6-OAU and lauric acid, alter Brucella abortus 544 infection in both in vitro and in vivo systems. Microb Pathog. 2021 Sep. 158:105079.

溶解性：

soluble in DMSO

保存條件：

-20℃

配置溶液濃度參考：

	1mg	5mg	10mg
1 mM	4.179 ml	20.893 ml	41.786 ml
5 mM	0.836 ml	4.179 ml	8.357 ml
10 mM	0.418 ml	2.089 ml	4.179 ml
50 mM	0.084 ml	0.418 ml	0.836 ml

注意：

部分產品我司僅能提供部分信息，我司不保證所提供信息的權威性，僅供客戶參考交流研究之用。

參考文獻

質檢證書(COA)

輸入產品批號：

如何獲取質檢證書(COA)?

請輸入貨號和一個與之匹配的批號。
例如:
批號：JS298415 貨號：S20001-25g
在貨品標簽上如何找到貨號和批號?

摩爾濃度計算器

質量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)

質量

濃度

體積

分子摩爾量

計算重置

玖玖资源网站,爆乳久久久,日韩精品乱码久久久蜜桃,精品呦国产一区二区三区,国产伦高清一区二区三区,sese一区二区,午夜精品福利一区二区三区,三级黄色网站久久免费