| 產品描述： | Angiotensin 1-7, also known as Ang-(1-7), is a synthetic heptapeptide identical to endogenous angiotensin-(1-7) , inhibits purified canine ACE activity (IC50=0.65 μM) with vasodilator and antiproliferative activities. |
| 靶點： |
Endogenous Metabolite;RAAS;RAAS;EndogenousMetabolite |
| 體內研究： |
Ang-(1-7) treatment attenuated the shortening of the atrial effective refractory period and the increase in the atrial fibrillation inducibility level. Immunohistochemistry and Western blotting showed sympathetic nerve hyperinnervation in the pacing group, while Ang-(1-7) attenuated sympathetic nerve proliferation. Ang-(1-7) alleviated the pacing-induced increases in tyrosine hydroxylase and nerve growth factor mRNA expression levels |
| 細胞實驗： |
Cultured cardiomyocytes were treated with PBS (control), ANG II (100 nM), ANG-(1–7) (100 nM), ANG II + ANG-(1–7), ANG II + ANG-(1–7) + A779 (10 μM), ANG II + ANG-(1–7) + Sirt3 short hairpin (sh)RNA, lentiviral particles (1 × 10^6 TU) containing a Sirt3 shRNA viral vector, or ANG II + Mito-TEMPO (0.1 μM) for 24 h. Each treatment was performed in triplicate wells. Monoclonal anti-α-sarcomeric actin antibody was used for identification and measurement of cardiomyocytes via immunofluorescence staining. Cells were fixed in methanol at 10°C and washed with fresh washing solution (0.1% Triton X-100 in PBS) three times. After preincubation with 3% BSA for 20 min, cells were incubated with primary antibody, which was diluted to 1:100 with PBS containing 3% BSA, overnight at 4°C. After three washings with the washing solution, cells were incubated with fluorescence-conjugated anti-mouse IgG (1:500 diluted in PBS) for 1 h at room temperature. Photographic images of cardiomyocytes were taken with a fluorescence microscop |
| 動物實驗： |
Eighteen dogs were randomly assigned to sham group, pacing group and Ang-(1-7) group. Rapid atrial pacing was maintained for 14 days in the pacing and Ang-(1-7) groups. Ang-(1-7) was administered intravenously in the Ang-(1-7) group. The atrial effective refractory period and atrial fibrillation inducibility level were measured at baseline and under sympathetic nerve stimulation after 14 days of measurement. The atrial sympathetic nerves labeled with tyrosine hydroxylase were detected using immunohistochemistry and Western blotting, and tyrosine hydroxylase and nerve growth factor mRNA levels were measured by reverse transcription polymerase chain reaction |
| 參考文獻： |
1.Guo L , Yin A , Zhang Q , et al. Angiotensin (1-7) Attenuates Angiotensin II-induced Cardiac Hypertrophy via a Sirt3-Dependent Mechanism[J]. American Journal of Physiology - Heart and Circulatory Physiology, 2017:ajpheart.00768.2016. 2.Shangguan W , Shi W , Li G , et al. Angiotensin-(1–7) attenuates atrial tachycardia-induced sympathetic nerve remodeling[J]. Journal of the Renin-Angiotensin-Aldosterone System, 2017, 18(3):147032031772928. |
| 溶解性： |
Soluble in H2O |
| 保存條件： |
-20°C |
| 配置溶液濃度參考： |
|
1mg |
5mg |
10mg |
| 1 mM |
1.112 ml |
5.562 ml |
11.123 ml |
| 5 mM |
0.222 ml |
1.112 ml |
2.225 ml |
| 10 mM |
0.111 ml |
0.556 ml |
1.112 ml |
| 50 mM |
0.022 ml |
0.111 ml |
0.222 ml |
|
| 注意： |
部分產品我司僅能提供部分信息，我司不保證所提供信息的權威性，僅供客戶參考交流研究之用。 |
上海工商
危險品化學品經營許可證（不帶存儲）
營業執照(三證合一)
北京