貨號	規格	價格	上海	北京	武漢	南京
S81180-5mg	98.4%	￥544.00 S81180-5mg ￥544.00 現貨	6 6	-	-	-
S81180-10mg	98.4%	￥829.60 S81180-10mg ￥829.60 現貨	7 7	-	-	-
S81180-25mg	98.4%	￥1224.00 S81180-25mg ￥1224.00 現貨	6 6	-	-	-
S81180-100mg	98.4%	￥3264.00 S81180-100mg ￥3264.00 現貨	7 7	-	-	-

產品介紹

FLAG peptide is an eight amino acids peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) with an enterokinase-cleavage site; designed for antibody-mediated identification and purification of recombinant proteins.

產品描述：

靶點：

Peptides;Others

體外研究：

Fusion protein technology has become an important tool for solving numerous problems linked to recombinant protein production. The properties of the additional tag facilitate identification and provide a one-step purification procedure of the fusion protein by passing cell extracts or supernatants through columns of an appropriate matrix. FLAG peptide allows elution under non-denaturing conditions. Several antibodies against FLAG peptide have been developed. One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAGe peptide. Antibodies M2 and M5 are applied in this procedure. The Flag-tag is first described as a calcium-dependent epitope of a monoclonal antibody. It is a highly acidic octapeptide which can be N-terminally fused to the protein of interest. As a very hydrophilic peptide the Flag–tag has a high surface probability. Flag-fusion proteins can be captured by an immunoaffinity column in the presence of Ca2+ and eluted byEDTA at low concentrations, neutral pH and thus, nearly physiological conditions。

參考文獻：

1. Einhauer A, et al. The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 2001 Oct 30;49(1-3):455-65.
2. Schuster M, et al. Protein expression in yeast; comparison of two expression strategies regarding proteinmaturation. J Biotechnol. 2000 Dec 28;84(3):237-48.

溶解性：

Soluble in H2O

保存條件：

-20℃

配置溶液濃度參考：

	1mg	5mg	10mg
1 mM	0.987 ml	4.936 ml	9.872 ml
5 mM	0.197 ml	0.987 ml	1.974 ml
10 mM	0.099 ml	0.494 ml	0.987 ml
50 mM	0.02 ml	0.099 ml	0.197 ml

注意：

部分產品我司僅能提供部分信息，我司不保證所提供信息的權威性，僅供客戶參考交流研究之用。

參考文獻

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FLAG Peptide TFA salt

DYKDDDDK Peptide TFA salt