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轉鐵蛋白

    
BR,98%

TRF

源葉
S12027 一鍵復制產品信息
11096-37-0
MFCD00131337
鐵傳遞蛋白
貨號 規格 價格 上海 北京 武漢 南京 購買數量
S12027-10mg BR,98% ¥120.00 2 - 1 3
S12027-50mg BR,98% ¥380.00 >10 1 - 2
S12027-100mg BR,98% ¥700.00 >10 2 6 1
S12027-500mg BR,98% ¥2800.00 3 1 - 3
產品介紹 參考文獻(51篇) 質檢證書(COA) 摩爾濃度計算器 相關產品

產品介紹

介紹:轉鐵蛋白是血漿中主要的鐵傳遞蛋白,為細胞分化和細胞代謝提供所需的鐵。研究發現 所有的細胞生長都離不開轉鐵蛋白。不但維持正常的細胞代謝依賴于以生物活性形態存在的 鐵,鐵還是一些酶的輔助因子,比如 RNA 聚合酶,DNA 合成酶。同時也是血紅蛋白的重要 組成部分。

轉鐵蛋白是血漿糖蛋白,從血漿中分離而來,牛轉鐵蛋白以兩種分子形態存在,分子量 分別為 74KD 和 78KD。每個轉鐵蛋白分子通過 C 端和 N 端結構位點可以結合兩分子鐵離子, 所以轉鐵蛋白以三種形態存在:脫鐵(不結合鐵離子),飽和鐵(結合兩分子鐵離子)和部 分飽和,在生理情況下,轉鐵蛋白分子僅有 1/3 被鐵飽和。

使用說明: 轉鐵蛋白對于絕大部分細胞系在無血清培養基中生長是必需的,研究表明飽和鐵形態的 轉鐵蛋白對于促進細胞生長是最有效的形態。對于雜交瘤,黏連細胞和懸浮細胞系,在細胞 培養過程中,應保持培養基中轉鐵蛋白濃度在 0.5-100μ g/ml。針對不同的細胞系,需要選 擇最合適的濃度。

轉鐵蛋白凍干粉需要保存在冷藏狀態下,使用之前再溶解。一般用 PBS 或者其它合適 的溶劑在室溫下將轉鐵蛋白凍干粉溶解成儲液,濃度在 0.5-2.0mg/ml,然后用無菌濾膜進行過 濾,濾液可在無菌條件下 2-8℃保存 30 天。

外觀: 凍干粉末
儲存條件: 2-8℃
注意: 部分產品我司僅能提供部分信息,我司不保證所提供信息的權威性,僅供客戶參考交流研究之用。

參考文獻(51篇)

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摩爾濃度計算器

質量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)

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