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丙二醛(MDA)檢測試劑盒(TBA微板法)

    

源葉
R21869 一鍵復制產品信息
貨號 規格 價格 上海 北京 武漢 南京 購買數量
R21869-100T
¥310.00 7 - - -
產品介紹 參考文獻(36篇) 質檢證書(COA) 摩爾濃度計算器 相關產品

產品介紹

動物或植物細胞發生氧化應激(oxidative stress)時,會發生脂質氧化。丙二醛(Malondialdehyde, MDA)是一種生物體脂質氧化的天然產物,一些脂肪酸氧化后逐漸分解為一系列包括MDA在內的復雜化合物,此時通過檢測MDA的水平即可檢測脂質氧化的水平,因此MDA的測定被廣泛用作脂質氧化的指標。生物體內的一些其它生化反應也會產生MDA,例如thromboxane synthase也可以催化產生,但只要在測定時設置適當對照即可觀察到脂質氧化水平的變化。

本司丙二醛(MDA)檢測試劑盒(TBA微板法,MDA Assay Kit)又稱脂質氧化(MDA)檢測試劑盒,是采用一種基于MDA和硫代巴比妥酸(thiobarbituric acid, TBA)反應產生紅色產物的顯色反應,隨后通過比色法用于對血漿、血清、尿液、動植物組織或細胞裂解液中MDA進行定量檢測,廣泛用于脂質氧化(lipid peroxidation)水平檢測,丙二醛在較高溫度及酸性環境中可與TBA發生反應形成紅色的MDA-TBA加合物,MDA-TBA加合物在535nm處有最大吸收,據此可以通過比色法進行檢測。另外MDA-TBA加合物也可以在535nm被激發產生最大發射波長553nm,據此也可以進行熒光檢測。本試劑盒僅用于科研領域,不宜用于臨床診斷或其他用途。

注意事項:利用丙二醛(MDA)與硫代巴比妥酸(TBA)結合產生紅色復合物,酶標儀檢測532nm處吸光度,進而計算出MDA含量,反應出脂質氧化情況。。

儲存條件: -20℃,避光
有效期: 12個月
用途: 本品僅供科研,不得用于其它用途。
注意: 部分產品我司僅能提供部分信息,我司不保證所提供信息的權威性,僅供客戶參考交流研究之用。

參考文獻(36篇)

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摩爾濃度計算器

質量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)

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